Composite

Part:BBa_K2360007:Design

Designed by: HU RIRONG   Group: iGEM17_SCUT-China_A   (2017-10-27)


pChrB-ChrB


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 136
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 911
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We combined with the rrnb' terminator for a convenient using.


Source

We synthesized this part, optimized for E.coil K12.

References

[1]. Branco R, Morais P V. Identification and characterization of the transcriptional regulator ChrB in the chromate resistance determinant of Ochrobactrum tritici 5bvl1 [J]. Plos One, 2013, 8(11):e77987-e77987.